Comparison of Quantitative Fecal Examination Using the Stoll Method and the Kato-Katz Method in Detecting the Number of Soil-Transmitted Helminth Worm Eggs
Keywords:
Soil-Transmitted Helminths, Stoll Method, Kato-Katz Method, Helminth Eggs, Stool ExaminationAbstract
Soil-Transmitted Helminths (STH) are a group of parasitic worms transmitted through contaminated soil, with prevalence remaining high, particularly among elementary school-aged children. Detection of STH eggs through microscopic stool examination can be performed using the Stoll and Kato-Katz methods. Although both are quantitative methods frequently used, a comparative analysis of their detection results requires further investigation. To compare the number of STH eggs detected using the Stoll method and the Kato-Katz method in stool samples from elementary school students. This research was an analytical study with a cross-sectional design. A total of 67 stool samples from students at SDN 101931 Perbaungan were examined using the Stoll and Kato-Katz methods. Data were analyzed using the Wilcoxon test. Most samples tested negative for STH infection. The Kato-Katz method detected four positive cases (6.0%), while the Stoll method detected one positive case (1.5%). The types of worms detected using the Kato-Katz method were Ascaris lumbricoides and Trichuris trichiura, with average egg counts of 750 and 37.5 eggs per gram of stool (EPG), respectively. The Stoll method detected only Ascaris lumbricoides, with an average egg count of 200 EPG. The Wilcoxon test showed no statistically significant difference in the number of Ascaris lumbricoides eggs detected by the Kato-Katz and Stoll methods (p = 0.068). No statistically significant difference was found in the number of Ascaris lumbricoides eggs detected by the Stoll and Kato-Katz methods. However, the Kato-Katz method detected more positive samples and both STH species identified in this study, whereas the Stoll method detected only Ascaris lumbricoides. These findings suggest that the Kato-Katz method may provide greater detection yield under the conditions of this study, while the Stoll method may serve as an alternative for quantitative stool examination.
References
Abelira R, Mutiara H. Perbandingan pemeriksaan tinja metode sedimentasi formol-ether dengan metode Kato-Katz dalam mendeteksi Soil-Transmitted Helminth. Medula. 2023;13:463–471.
Arifta RH, Suhartini, Makkadafi SP. Studi deskriptif pemeriksaan efektivitas sampel feses metode langsung dan sedimentasi telur STH (Soil-Transmitted Helminth). Borneo Journal of Science and Mathematics Education. 2022;2(3).
Barda B, Schindler C, Wampfler R, Ame SM, Ali SM, Keiser J. Comparison of real-time PCR and the Kato-Katz method for the diagnosis of soil-transmitted helminthiasis and assessment of cure in a randomized controlled trial. BMC Microbiology. 2020;20(1):1–8. doi:10.1186/s12866-020-01963-9.
Bartlett AW, Mendes EP, Dahmash L, et al. Knowledge, attitudes, practices and acceptability of a school preventive chemotherapy programme for schistosomiasis and soil-transmitted helminths control in Angola. Philosophical Transactions of the Royal Society B: Biological Sciences. 2023;378(1887). doi:10.1098/rstb.2022.0430.
Bosch F, Palmeirim MS, Ali SM, et al. Diagnosis of soil-transmitted helminths using the Kato-Katz technique: What is the influence of stirring, storage time and storage temperature on stool sample egg counts? PLoS Neglected Tropical Diseases. 2021;15(1):1–17. doi:10.1371/journal.pntd.0009032.
Coffeng LE, Vlaminck J, Cools P, et al. A general framework to support cost-efficient fecal egg count methods and study design choices for large-scale STH deworming programs—monitoring of therapeutic drug efficacy as a case study. PLoS Neglected Tropical Diseases. 2023;17(5):1–23. doi:10.1371/journal.pntd.0011071.
Devi S. Uji Perbandingan Jumlah Telur Cacing Soil-Transmitted Helminth Menggunakan Metode Stoll dengan Metode Kato-Katz. Repositori Universitas Perintis Indonesia. 2020:1–38.
Dewi SM. Artikel Prodi Sarjana Terapan Teknologi Laboratorium Medis. 2021.
Garcia L. Diagnostic Medical Parasitology. 5th ed. 2015.
Kabatende J, Mugisha M, Ntirenganya L, et al. Prevalence, intensity, and correlates of soil-transmitted helminth infections among school children after a decade of preventive chemotherapy in Western Rwanda. Pathogens. 2020;9(12):1–20. doi:10.3390/pathogens9121076.
Kementerian Kesehatan Republik Indonesia. Peraturan Menteri Kesehatan Republik Indonesia Nomor 15 Tahun 2017 tentang Penanggulangan Kecacingan. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017.
Khurana S, Singh S, Mewara A. Diagnostic techniques for soil-transmitted helminths—recent advances. Research and Reports in Tropical Medicine. 2021;12:181–196. doi:10.2147/rrtm.s278140.
Lestari D. Infeksi Soil-Transmitted Helminths pada anak. Science Journal. 2022;1(6):423–433. doi:10.56260/sciena.v1i6.75.
Lebu S, Kibone W, Muoghalu CC, et al. Soil-transmitted helminths: A critical review of the impact of co-infections and implications for control and elimination. PLoS Neglected Tropical Diseases. 2023;17(8):1–21. doi:10.1371/journal.pntd.0011496.
Matamoros G, Sanchez A, Cimino R, Krolewiecki A, Mejia R. A comparison of the diagnostic capability of Kato-Katz and real-time PCR for the assessment of treatment efficacy of ivermectin and albendazole combination against T. trichiura infections. PLoS Neglected Tropical Diseases. 2024;18(11):e0012677. doi:10.1371/journal.pntd.0012677.
Mrimi EC, Welsche S, Ali SM, Hattendorf J, Keiser J. Emodepside for Trichuris trichiura and hookworm infection. The New England Journal of Medicine. 2023;388(20):1863–1875. doi:10.1056/NEJMoa2212825.
Ngwese MM, Manouana GP, Moure PAN, Ramharter M, Esen M, Adégnika AA. Diagnostic techniques of soil-transmitted helminths: Impact on control measures. Tropical Medicine and Infectious Disease. 2020;5(2). doi:10.3390/tropicalmed5020093.
Nikmatullah NA, Wijiastuti W, Riyanti HB, et al. Pengabdian masyarakat melalui edukasi pencegahan dan pengobatan infeksi kecacingan di Cabang Aisyiyah Pasar Minggu. EJOIN: Jurnal Pengabdian Masyarakat. 2023;1(11):1315–1319. doi:10.55681/ejoin.v1i11.1791.
Schlosser-Brandenburg J, Midha A, Mugo RM, et al. Infection with soil-transmitted helminths and their impact on coinfections. Frontiers in Parasitology. 2023;2:1–18. doi:10.3389/fpara.2023.1197956.
Setiawan B, Ayu G, Syayyidah D, Hardisari R, Tri Widada S, Nuryati A. Jumlah telur cacing Soil-Transmitted Helminth (STH) pada metode sedimentasi dan flotasi. Jurnal Kesehatan Lingkungan. 2022;12(1):142–145. doi:10.47718/jkl.v10i2.1184.
Sinky AR. Kepadatan Telur Cacing Soil-Transmitted Helminths Memakai Metode Stoll pada Anak Usia 5–12 Tahun di Daerah Kampung Jambak. Program Studi Diploma III Teknologi Laboratorium Medis, Universitas Perintis Indonesia. 2024.
Stuyver LJ, Levecke B. The role of diagnostic technologies to measure progress toward WHO 2030 targets for soil-transmitted helminth control programs. PLoS Neglected Tropical Diseases. 2021;15(6):1–7. doi:10.1371/journal.pntd.0009422.
Taquillah NS, Mahtuti EY, Masyhur M, Faisal. Identification of soil-transmitted helminth using formol ether sedimentation and ZnSO4 solution flotation methods. Medicra: Journal of Medical Laboratory Science. 2022;5(2):68–73. doi:10.21070/medicra.v5i2.1634.
Ummah MS. Introduction to Real Analysis. Vol. 11. 2019.
Vegvari C, Giardina F, Malizia V, De Vlas SJ, Coffeng LE, Anderson RM. Impact of key assumptions about the population biology of soil-transmitted helminths on the sustainable control of morbidity. Clinical Infectious Diseases. 2021;72(Suppl 3):S188–S194. doi:10.1093/cid/ciab195.
Wang J, Davis RE. Ascaris. Current Biology. 2020;30(10):R423–R425. doi:10.1016/j.cub.2020.02.064.
Zendejas-Heredia PA, Colella V, Hii SF, Traub RJ. Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stool. PLoS Neglected Tropical Diseases. 2021;15(5):1–18. doi:10.1371/journal.pntd.0009395.
